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PubMed · 1726650

Partial characterization of human amniotic membrane interferon.

P C Ferreira·C Gonzaga·E G Kroon·J R Dos-Santos·H M Bicalho·R R Golgher
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Abstract

1. The molecular weight and the electrofocusing profile of human amniotic membrane interferon (IFN-AM) were determined. 2. When submitted to gel filtration, IFN-AM showed a single 26-28 kDa component; in polyacrylamide gel electrophoresis one component of 19,500. 3. In electrofocusing, IFN-AM displayed a heterogeneous profile with 5 to 7 peaks, but different from human alpha or beta IFNs. This heterogeneity was reduced by previous treatment of IFN-AM with neuraminidase. 4. IFN-AM is a sialoglycoprotein similar to human beta IFN in terms of antigenicity but different from it in electrofocusing profile.

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Chromatography, GelElectrophoresis, Polyacrylamide GelExtraembryonic MembranesHumansInterferonsIsoelectric FocusingMolecular WeightPlacentaJournal ArticleResearch Support, Non-U.S. Gov't

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P C Ferreira, C Gonzaga, E G Kroon, J R Dos-Santos, H M Bicalho, R R Golgher. 1991. Partial characterization of human amniotic membrane interferon.. https://pubmed.ncbi.nlm.nih.gov/1726650/

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