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Phosphoprotein phosphatase activity in the thyroid.

S W Spaulding·G N Burrow
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Abstract

Phosphoprotein phosphatase activity in the calf thyroid was found in various subcellular fractions. The relative amount in each fraction varied according to the substrate used: The 500g fraction had the highest specific activity when protamine was used, while the 5000g fraction was highest when histone was used. Triton X-100 tended to increase activity in all the particulate fractions, the greatest change being found in the 105,000g pellet. DEAE chromatography of the 105,000 g supernatant resolved at least three peaks of phosphoprotein phosphatase activity.

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AnimalsCattleCell FractionationChromatography, DEAE-CelluloseHistonesMembranesPhosphoric Monoester HydrolasesProtaminesThyroid GlandJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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S W Spaulding, G N Burrow. 1975. Phosphoprotein phosphatase activity in the thyroid.. https://doi.org/10.3181/00379727-150-39080

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