Platelet-derived endothelial cell growth factor: structure and function.

K Miyazono; F Takaku

doi:10.1253/jcj.55.1022

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Platelet-derived endothelial cell growth factor: structure and function.

K Miyazono·F Takaku

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Abstract

Platelet-derived endothelial cell growth factor (PD-ECGF) is a 45-kDa single chain polypeptide, which stimulates the growth and chemotaxis of endothelial cells in vitro and angiogenesis in vivo. Purification from human platelets and cDNA cloning of PD-ECGF disclosed that it is a novel type of angiogenic factor without sequence similarity to hitherto known proteins. PD-ECGF is present in human platelets as well as in placenta. Amino acid sequencing of PD-ECGF from human placenta revealed that the placental form has an additional 5 amino acids at the N-terminus. In cultured cells, it is produced by normal fibroblasts as well as some transformed cell lines. PD-ECGF lacks a hydrophobic signal sequence and remains inside the producer cells. PD-ECGF may act at sites of injury as a wound hormone and thus play an important role under several physiological and pathological conditions.

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Blood Platelets Cell Division Cloning, Molecular Endothelium, Vascular Female Gene Expression Humans Molecular Structure Placenta Pregnancy Thymidine Phosphorylase Journal Article Review

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K Miyazono, F Takaku. 1991. Platelet-derived endothelial cell growth factor: structure and function.. https://doi.org/10.1253/jcj.55.1022

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