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PubMed · 1750669

Biotinylated basic fibroblast growth factor is biologically active.

I Pieri·D Barritault
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Abstract

Basic fibroblast growth factor (bFGF) was modified by biotinylation via amino group substitution, using biotin-N-hydroxysuccinimide ester at molar reaction ratios of 20, 200, and 2000 per bFGF molecule (respectively named bio-bFGF.20, bio-bFGF.200, and bio-bFGF.2000). The biotinylated bFGF derivatives, bio-bFGF.20 and bio-bFGF.200, conserved the same affinity for heparin as native bFGF, in contrast to bio-FGF.2000 which lost this property. Bio-bFGF.20 and bio-bFGF.200 were as effective as native bFGF in their capacity to compete with 125I-bFGF for binding to bFGF receptor on bovine brain membranes. The biological activity of these bFGF derivatives was tested on CCL39 cells; bio-bFGF.20 and bio-bFGF.200 were as able as native bFGF to promote growth of CCL39.

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BiotinFibroblast Growth Factor 2Radioligand AssaySuccinimidesJournal ArticleResearch Support, Non-U.S. Gov't

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I Pieri, D Barritault. 1991. Biotinylated basic fibroblast growth factor is biologically active.. https://doi.org/10.1016/0003-2697(91)90319-o

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