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PubMed · 1751406

Decrease of proliferation rate and induction of differentiation by a MYCN antisense DNA oligomer in a human neuroblastoma cell line.

A Negroni·S Scarpa·A Romeo·S Ferrari·A Modesti·G Raschellà
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Abstract

The effects of an antisense oligodeoxynucleotide to codons 2-7 of the oncogene MYCN on the human neuroblastoma cell line LAN-5 were studied. Treated cells showed a decreased MYCN protein expression and synthesis by immunoperoxidase staining and immunoprecipitation. At the same time, the replication rate was inhibited, and the phenotype was modified toward a more differentiated type. Our data suggest the involvement of oncogene MYCN in both proliferative and differentiative processes.

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Base SequenceCell DifferentiationCell DivisionCell Line, TransformedDNA ReplicationDNA, AntisenseGene Expression Regulation, NeoplasticGenes, mycHumansMolecular Sequence DataNeurofilament ProteinsVimentinJournal ArticleResearch Support, Non-U.S. Gov't

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A Negroni, S Scarpa, A Romeo, S Ferrari, A Modesti, G Raschellà. 1991. Decrease of proliferation rate and induction of differentiation by a MYCN antisense DNA oligomer in a human neuroblastoma cell line.. https://pubmed.ncbi.nlm.nih.gov/1751406/

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