Phospholipid acylation by mouse sciatic nerve microsomes.

C Cassagne; H Juguelin; F Boiron

doi:10.1016/0005-2736(91)90153-y

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PubMed · 1751518

Phospholipid acylation by mouse sciatic nerve microsomes.

C Cassagne·H Juguelin·F Boiron

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Abstract

The partition of 0.3 nmol of [1-14C]oleoyl-CoA in the microsomes (10 micrograms proteins) from mouse sciatic nerves is unaffected by the presence of lysophospholipids and is about 45% of the total oleoyl-CoA (77% of the acylglycerophosphocholine partition in the membrane). The concentration of both oleoyl-CoA and acylglycerophosphocholine is over 1 mM in the membrane. There is a selective acyl transfer from acyl-CoA to lysolipid acceptors (oleoyl greater than myristoyl, palmitoyl, stearoyl much greater than eicosanoyl greater than docosanoyl, tetracosanoyl). The exogenous acyl acceptors are acylglycerophosphocholine and acylglycerophosphoinositol and to a lesser extent acylglycerophosphoethanolamine, but not acylglycerophosphoserine. A PC formation from acylGPC in the absence of exogenous acyl donors or from oleoyl-CoA in the absence of exogenous acyl acceptor was also observed.

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Acylation Animals Catalysis Cell Membrane In Vitro Techniques Mice Microsomes Phospholipids Sciatic Nerve Journal Article

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C Cassagne, H Juguelin, F Boiron. 1991-11-18. Phospholipid acylation by mouse sciatic nerve microsomes.. https://doi.org/10.1016/0005-2736(91)90153-y

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