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Transcriptional termination between the closely linked human complement genes C2 and factor B: common termination factor for C2 and c-myc?

R Ashfield·P Enriquez-Harris·N J Proudfoot
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Abstract

We have demonstrated, using a combination of nuclear run-off and poly(A) site competition assays, that transcriptional termination occurs between the closely spaced human complement genes, C2 and Factor B, soon after the C2 poly(A) site. A comparison of the C2 termination signal with a functionally similar sequence downstream of the human alpha 2 globin gene reveals that both signals function in an orientation dependent manner, with subfragments of the whole signal displaying partial effects. In the case of the C2 termination sequence a protein binds within it, and is partially responsible for the termination effect. We further demonstrate that the same (or closely related) protein binds to the ME1a1 site in the murine c-myc promoter, which has been implicated in c-myc attenuation. We suggest that the termination/pause sequences positioned downstream of a gene's poly(A) site may constitute the general signals that elicit transcriptional termination in genes transcribed by RNA polymerase II.

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Base SequenceChloramphenicol O-AcetyltransferaseComplement C2Complement Factor BDNA FingerprintingGlobinsHumansLiverMolecular Sequence DataPlasmidsPromoter Regions, GeneticProto-Oncogene Proteins c-mycRestriction MappingSequence Homology, Nucleic AcidTerminator Regions, GeneticTransfectionbeta-GalactosidaseJournal ArticleResearch Support, Non-U.S. Gov't

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R Ashfield, P Enriquez-Harris, N J Proudfoot. 1991. Transcriptional termination between the closely linked human complement genes C2 and factor B: common termination factor for C2 and c-myc?. https://doi.org/10.1002/j.1460-2075.1991.tb04998.x

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