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PubMed · 1762915

An efficient, sequence-specific method for crosslinking complementary oligonucleotides using binuclear platinum complexes.

E S Gruff·L E Orgel
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Abstract

The binuclear PtII complexes [(trans-Pt(NH3)2Cl)2 (NH2(CH2)nNH2)]Cl2 (n = 4, 5 or 6), crosslink oligodeoxynucleotide-5'-phosphorothioates rapidly, specifically and efficiently to complementary single-stranded oligodeoxynucleotide targets. In the case that we investigated in detail, the most abundant crosslink is formed to the G residue complementary to the 5'-terminal C residue of the phosphorothioate. Less efficient crosslinking occurs to many other residues of the target. The same PtII complexes also bring about crosslinking efficiently to the polypurine tract in triple-helical DNA.

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Base CompositionBase SequenceCross-Linking ReagentsElectrophoresisMolecular Sequence DataNucleic Acid ConformationOligodeoxyribonucleotidesPlatinumJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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E S Gruff, L E Orgel. 1991-12-25. An efficient, sequence-specific method for crosslinking complementary oligonucleotides using binuclear platinum complexes.. https://doi.org/10.1093/nar%2F19.24.6849

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