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PubMed · 1765644

A novel method for synchronizing a B cell lymphoma.

P C Isakson·J Purkerson·K Catron·T P Bender
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Abstract

Certain sub-lines of the murine B cell lymphoma BCL1 can be maintained in vitro and respond to cytokines including IL-2 and IL-5. BCL1 cells, as well as other B lymphomas, are difficult to synchronize using conventional techniques such as thymidine block or DNA synthesis inhibition. We have found that BCL1 cells maintained in Dulbecco's minimum essential medium (DMEM) with non-essential amino acids (NEAA) can be readily synchronized by culture in DMEM lacking NEAA. Within 10-18 h of medium replacement, 98% of BCL1 cells are 2 N in DNA content, suggesting that these cells are arrested in G0/G1. This population of BCL1 cells is viable and can be stimulated to enter S phase by culture in media containing NEAA; however, arrested cells did not appear to return synchronously into the cell cycle on addition of NEAA. A transient increase in levels of c-fos and c-myc mRNA was not detected after arrested BCL1 cells were stimulated to enter S phase, suggesting that arrested cells are in the G1 phase of the cell cycle, rather than G0. This technique for obtaining G1 arrested B lymphoma cells may prove useful in the analysis of molecular events that occur in B cells as a function of cell cycle position.

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Amino AcidsCell CycleGene ExpressionGenes, fosGenes, mycHumansIn Vitro TechniquesIsoleucineLymphoma, B-CellProto-Oncogene Proteins c-fosProto-Oncogene Proteins c-mycTumor Cells, CulturedJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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P C Isakson, J Purkerson, K Catron, T P Bender. 1991-12-15. A novel method for synchronizing a B cell lymphoma.. https://doi.org/10.1016/0022-1759(91)90319-b

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