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PubMed · 1770041

Changes in glomerular extracellular matrices components in diabetic nephropathy.

S Ikeda·H Makino·T Haramoto·K Shikata·I Kumagai·Z Ota
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Abstract

The changes in glomerular extracellular matrices components in diabetic nephropathy were investigated. Indirect immunofluorescence staining, using polyclonal antibodies to heparan sulfate proteoglycan (HS-PG), laminin, type IV collagen, and fibronectin was carried out on renal specimens obtained by needle biopsy. Immunofluorescence intensity and distribution were observed. HS-PG and laminin decreased in the capillary walls; on the other hand, type IV collagen and fibronectin tended to increase in the mesangial area. HS-PG and laminin decreased in inverse proportion to sclerosis grades and proteinuria. These changes seemed to play an important role in progression of diabetic glomerulosclerosis.

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Biopsy, NeedleCollagenDiabetes Mellitus, Type 1Diabetes Mellitus, Type 2Diabetic NephropathiesExtracellular MatrixFemaleFibronectinsFluorescent Antibody TechniqueHeparan Sulfate ProteoglycansHeparan SulfateHumansKidney GlomerulusLamininMaleMiddle AgedProteoglycansJournal ArticleResearch Support, Non-U.S. Gov't

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S Ikeda, H Makino, T Haramoto, K Shikata, I Kumagai, Z Ota. Changes in glomerular extracellular matrices components in diabetic nephropathy.. https://doi.org/10.1016/0891-6632(91)90068-z

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Biopsy, Needle↗