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PubMed · 1782334

Thrombin-hirudin complex stability: a comparison with the thrombin-antithrombin III complex.

J Römisch·E P Pâques
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Abstract

In the present in vitro study the stabilities of the thrombin-hirudin and the thrombin-antithrombin III complexes were investigated. After incubation of the complexes with free inhibitors the thrombin-antithrombin III levels were determined by ELISA. The thrombin-hirudin complex proved to be stable in the presence of antithrombin III or heparin. However, in the presence of heparin and plasma equivalent concentrations of antithrombin III, the thrombin-hirudin complex dissociated and hirudin was displaced. In contrast, both thrombin-antithrombin III and thrombin-antithrombin III/heparin complexes are very stable even in the presence of a large excess of hirudin.

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Antithrombin IIIDrug StabilityHeparinHirudinsHumansPeptide HydrolasesThrombinComparative StudyJournal Article

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J Römisch, E P Pâques. 1991. Thrombin-hirudin complex stability: a comparison with the thrombin-antithrombin III complex.. https://pubmed.ncbi.nlm.nih.gov/1782334/

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