Avoiding gas bubble formation during freeze substitution.

D B Fisher

doi:10.3109/10520299109109982

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Avoiding gas bubble formation during freeze substitution.

D B Fisher

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Abstract

Gas bubbles frequently are formed during freeze substitution, especially when tissues are warmed to room temperature. The problem arises largely from the extreme solubility of CO2 in the freeze substitution solvent. Gas bubbles may be minimized by briefly transferring the tissue to freshly chilled solvent before warming to room temperature.

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Carbon Dioxide Chemical Phenomena Chemistry, Physical Frozen Sections Gases Temperature Journal Article

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D B Fisher. 1991. Avoiding gas bubble formation during freeze substitution.. https://doi.org/10.3109/10520299109109982

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