Comparison and modification of rRNA sequencing methods.

R F Wang; W W Cao; M F Slavik

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PubMed · 1793573

Comparison and modification of rRNA sequencing methods.

R F Wang·W W Cao·M F Slavik

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Abstract

In this study, modification of two methods of RNA sequencing resulted in more definitive sequencing bands. In one method of sequencing, the bands of lane A and lane G sometimes were not clear. Modifications of this method by changing the concentrations of ddATP and ddGTP resulted in the bands of lane A and lane G becoming more readable. Although a second sequencing method was found to have clearer bands than the first method, and the bases immediately downstream from the primer binding site could be read by using r-32P-labeled primer, the bands on the top of lane A still were not clear. Modifications of this second method by changing the ddATP/dATP ratio resulted in the bands of lane A becoming much clearer.

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Base Sequence Campylobacter jejuni DNA Genetic Techniques Molecular Sequence Data RNA, Ribosomal RNA, Ribosomal, 16S Comparative Study Journal Article Research Support, Non-U.S. Gov't

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R F Wang, W W Cao, M F Slavik. 1991. Comparison and modification of rRNA sequencing methods.. https://pubmed.ncbi.nlm.nih.gov/1793573/

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