Murine studies using polyethylene glycol-modified recombinant human interleukin 2 (PEG-IL-2): antitumor effects of PEG-IL2 alone and in combination with adoptive cellular transfer.

J C Yang; S L Schwarz; D M Perry-Lalley; S A Rosenberg

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PubMed · 1804310

Murine studies using polyethylene glycol-modified recombinant human interleukin 2 (PEG-IL-2): antitumor effects of PEG-IL2 alone and in combination with adoptive cellular transfer.

J C Yang·S L Schwarz·D M Perry-Lalley·S A Rosenberg

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Abstract

A polyethylene glycol-modified form of recombinant human IL-2 (PEG-IL-2) was tested for murine antitumor effects in vitro and in vivo. This PEG-IL-2 was demonstrated to retain the in vitro ability to support T cell proliferation, enhance a mixed lymphocyte reaction, and generate lymphokine-activated killer (LAK) cells. It was found to have a circulating half-life in mice 25 times longer than unmodified recombinant IL-2 (RIL-2). Serum levels were detected up to 60 h after a single intravenous injection. When given as a single, intravenous administration the antitumor effect of this material was similar to multiple, repeated bolus doses of RIL-2. PEG-IL-2 was also found to support the in vivo efficacy of adoptively transferred LAK cells and tumor infiltrating lymphocytes (TIL). Using a congenic TIL (Thy 1.1), persistence of adoptively transferred TIL was found to be prolonged with PEG-IL-2 compared to repeated boluses of RIL-2. Four days after transfer, twice as many Thy 1.1 TIL were recoverable from the lungs of mice given PEG-IL-2. These studies show that PEG-IL-2 is a modified lymphokine with significant antitumor activity in murine systems and is superior to bolus RIL-2 in enhancing the survival of adoptively transferred TIL.

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Animals Antineoplastic Agents Combined Modality Therapy Immunotherapy, Adoptive Injections, Intraperitoneal Injections, Intravenous Interleukin-2 Killer Cells, Lymphokine-Activated Lung Neoplasms Lymphocytes, Tumor-Infiltrating Mice Mice, Inbred C57BL Mice, Inbred DBA Polyethylene Glycols Recombinant Proteins Reference Values Journal Article

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J C Yang, S L Schwarz, D M Perry-Lalley, S A Rosenberg. 1991. Murine studies using polyethylene glycol-modified recombinant human interleukin 2 (PEG-IL-2): antitumor effects of PEG-IL2 alone and in combination with adoptive cellular transfer.. https://pubmed.ncbi.nlm.nih.gov/1804310/

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