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PubMed · 1821813

A two-step purification procedure for sheep liver 6-phosphogluconate dehydrogenase.

D O Somers·J Hajdu·M J Adams
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Abstract

A two-step procedure for the purification of 6-phosphogluconate dehydrogenase (EC 1.1.1.44; 6-PGDH) from sheep liver is described. The enzyme is directly bound to cellulose phosphate by batch extraction and eluted with a linear salt gradient. Purification is completed by affinity chromatography using NADP(+)-agarose. The result is 6-PGDH of high purity, greatly increased yield, and the highest specific activity yet achieved, with a significant reduction in the purification time.

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AnimalsCelluloseChromatography, AffinityChromatography, Ion ExchangeDialysisLiverPhosphogluconate DehydrogenaseSheepJournal ArticleResearch Support, Non-U.S. Gov't

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D O Somers, J Hajdu, M J Adams. A two-step purification procedure for sheep liver 6-phosphogluconate dehydrogenase.. https://doi.org/10.1016/1046-5928(91)90098-4

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