A simple reflectance microphotometer for dot immunobinding assay (dot-ELISA) quantification.

C S Ferreira; M U Ferreira

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PubMed · 1823264

A simple reflectance microphotometer for dot immunobinding assay (dot-ELISA) quantification.

C S Ferreira·M U Ferreira

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Abstract

The use of a simple reflectance microphotometer adapted to quantify enzymatic dot immunobinding assays is described. The instrument is versatile and precise by clinical laboratory standards (i.e., 1.1% coefficient of variation for ten consecutive readings of a grey cardboard). The instrument provides a low-cost alternative to commercially available equipment for quantitation of dot-ELISA assays.

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Animals Antibodies, Protozoan Enzyme-Linked Immunosorbent Assay Immunoblotting Immunoglobulin G Photometry Plasmodium falciparum Journal Article Research Support, Non-U.S. Gov't

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C S Ferreira, M U Ferreira. 1991. A simple reflectance microphotometer for dot immunobinding assay (dot-ELISA) quantification.. https://pubmed.ncbi.nlm.nih.gov/1823264/

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