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PubMed · 1825201

Abscisic acid induces a cytosolic calcium decrease in barley aleurone protoplasts.

M Wang·B Van Duijn·A W Schram
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Abstract

Cytosolic calcium concentrations (Cai) of barley aleurone protoplasts after stimulation with the plant hormone abscisic acid (ABA) were measured by using the calcium-sensitive fluorescent dye Indo-1. The measured basal Cai is about 200 nM. Stimulation with ABA induces a strong dose-dependent decrease in Cai to a minimal value of about 50 nM. This decrease occurs within 5 s. The Ca2+ antagonists La3+ and Cd2+ inhibit the ABA-induced Cai decrease in a dose-dependent manner, while the Ca2+ channel blockers verapamil and nifedipine give no inhibition. The induction of Cai decrease by ABA is consistent with activation of the plasma membrane Ca2(+)-ATPase by ABA. The possible role of this ABA-induced Cai decrease in ABA signal transduction and in counteracting the effects of gibberellic acid are discussed.

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Abscisic AcidCalciumCalcium-Transporting ATPasesCell MembraneCytosolEnzyme ActivationFluorescent DyesHordeumIndolesNifedipineProtoplastsVerapamilJournal ArticleResearch Support, Non-U.S. Gov't

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M Wang, B Van Duijn, A W Schram. 1991-01-14. Abscisic acid induces a cytosolic calcium decrease in barley aleurone protoplasts.. https://doi.org/10.1016/0014-5793(91)80086-i

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