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Diabetes-induced decrease in the mRNA coding for sarcoplasmic reticulum Ca(2+)-ATPase in adult rat cardiomyocytes.

M Russ·H Reinauer·J Eckel
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Abstract

The present study examined the level of the mRNA coding for the sarcoplasmic reticulum (SR) Ca(2+)-ATPase in isolated ventricular myocytes from streptozotocin-diabetic rats and genetically obese (fa/fa) rats using Northern blotting techniques. In both animal models one single transcript with a mean size of 4.12 kb could be detected. In insulin-deficient rats the Ca(2+)-ATPase transcript level decreased by 51% when compared to normal rats. In obese animals a modest decrease in the Ca(2+)-ATPase mRNA content to 77% of lean controls has been detected. Decreased mRNA expression of the SR Ca(2+)-ATPase may partly explain the delayed diastolic relaxation observed in the diabetic heart.

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AnimalsBlotting, NorthernCalcium-Transporting ATPasesCells, CulturedDNA ProbesDiabetes Mellitus, ExperimentalGene Expression Regulation, EnzymologicMaleMyocardiumRNA, MessengerRatsRats, Inbred StrainsReference ValuesSarcoplasmic ReticulumTranscription, GeneticJournal ArticleResearch Support, Non-U.S. Gov't

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M Russ, H Reinauer, J Eckel. 1991-08-15. Diabetes-induced decrease in the mRNA coding for sarcoplasmic reticulum Ca(2+)-ATPase in adult rat cardiomyocytes.. https://doi.org/10.1016/0006-291x(91)90977-f

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