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PubMed · 1839500

Screening recombinant DNA libraries: a rapid and efficient method for isolating cDNA clones utilizing the PCR.

N R Isola·H J Harn·D L Cooper
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Abstract

We describe an expeditious method for the isolation of cDNA clones utilizing PCR-based amplification of target sequences from cDNA libraries. This method is rapid, less labor-intensive and inexpensive when compared with screening libraries with radiolabeled probes. This method can be applied to isolate multiple members of a protein family as well as homologous genes in different species by designing appropriate primers to amplify the most conserved regions. Utilizing this method, a novel reticulocyte CD44 transcript was isolated.

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Bacteriophage lambdaBase SequenceDNAGene LibraryHumansMolecular Sequence DataOligonucleotide ProbesPolymerase Chain ReactionReceptors, Lymphocyte HomingJournal Article

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N R Isola, H J Harn, D L Cooper. 1991. Screening recombinant DNA libraries: a rapid and efficient method for isolating cDNA clones utilizing the PCR.. https://pubmed.ncbi.nlm.nih.gov/1839500/

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