Wee1(+)-like gene in human cells.

M Igarashi; A Nagata; S Jinno; K Suto; H Okayama

doi:10.1038/353080a0

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Wee1(+)-like gene in human cells.

M Igarashi·A Nagata·S Jinno·K Suto·H Okayama

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Abstract

The wee1+ gene is a mitotic inhibitor controlling the G2 to M transition of the fission yeast Schizosaccharomyces pombe and encodes a protein kinase with both serine- and tyrosine-phosphorylating activities. We have cloned a human gene (WEE1Hu) similar to wee1+ by transcomplementation of a yeast mutant. WEE1Hu encodes a protein homologous to the S. pombe wee1+ and mik1+ (a functionally redundant sibling of wee1+) kinases and effectively rescues a wee1 mutation. We report here that overexpression of WEE1Hu in fission yeast generates very elongated cells as a result of inhibition of the G2-M transition in the cell cycle. In addition, we detected a 3-kilobase-long WEE1Hu messenger RNA in all the human cell lines we examined. We conclude that a wee1(+)-like gene exists and is expressed in human cells.

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Amino Acid Sequence Base Sequence Cell Line Cloning, Molecular Gene Expression Humans Mitosis Molecular Sequence Data Protein Kinases RNA, Messenger Restriction Mapping Schizosaccharomyces Sequence Homology, Nucleic Acid Transfection Journal Article Research Support, Non-U.S. Gov't

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M Igarashi, A Nagata, S Jinno, K Suto, H Okayama. 1991-09-05. Wee1(+)-like gene in human cells.. https://doi.org/10.1038/353080a0

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