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PubMed · 184461

Incorporation of adenosine into ATP: formation of compartmentalized ATP.

E Rapaport·P C Zamecnik
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Abstract

The incorporation of [3H]adenosine, [3H]adenine, and [3H]hypoxanthine into adenine nucleotides of nude (athymic) mouse liver and human hepatoma grown subcutaneously in nude mice was studied. 3H and 32P radioactive labeling in vivo of acid-soluble nucleotides followed by chromatographic procedures indicated that, in contrast to [3H]adenine and [3H]hypoxanthine, [3H]adenosine is preferentially incorporated into ATP in comparison with its incorporation into AMP and ADP. This phenomenon, as well as complementing the recently reported 3-fold increase in total cellular ATP upon treatmen- with 0.5-1.0 mM concentrations of adenosine, indicates the formation from adenosine of compartmentalized ATP that is not produced from either adenine or hypoxanthine. The observed effect is of larger magnitude in the growth-arrested normal liver than in the actively growing tumor.

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AdenineAdenosineAdenosine DiphosphateAdenosine TriphosphateAnimalsCarcinoma, HepatocellularCell Transformation, NeoplasticHypoxanthinesLiverLiver NeoplasmsMiceMice, NudeNeoplasms, ExperimentalJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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BibTeXRIS

E Rapaport, P C Zamecnik. 1976. Incorporation of adenosine into ATP: formation of compartmentalized ATP.. https://doi.org/10.1073/pnas.73.9.3122

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