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PubMed · 1845798

L-type Ca2+ currents in ascidian eggs.

B Dale·R Talevi·L J DeFelice
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Abstract

We have studied Ca2+ currents in ascidian eggs using the whole-cell clamp technique. T and L components, as observed in somatic cells, are present and the L-type current predominates. Since the IV relationship for these inward currents overlap at -30 mV, separation of the two components using different voltage regimes is not feasible. Increasing external Ca2+ results in larger currents. The L-type current decreases in a dose-dependent fashion in the presence of Mn2+ and Nifedipine, while the T-type current is inhibited in Ni2+. When Ba2+ was used as the carrier ion, channel kinetics and conductance were completely altered. Considering the density and kinetics of L-type channels in unfertilized eggs it is probable they play an important role in regulating cytosolic Ca2+ during early developmental processes.

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AnimalsCalciumCalcium ChannelsElectric ConductivityMembrane PotentialsOocytesUrochordataComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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B Dale, R Talevi, L J DeFelice. 1991. L-type Ca2+ currents in ascidian eggs.. https://doi.org/10.1016/0014-4827(91)90190-6

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