Interactions between interleukin-1 and basic fibroblast growth factor on articular chondrocytes. Effects on cell growth, prostanoid production, and receptor modulation.

J E Chin; C A Hatfield; R F Krzesicki; W F Herblin

doi:10.1002/art.1780340309

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Interactions between interleukin-1 and basic fibroblast growth factor on articular chondrocytes. Effects on cell growth, prostanoid production, and receptor modulation.

J E Chin·C A Hatfield·R F Krzesicki·W F Herblin

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Abstract

The interactions of interleukin-1 beta (IL-1 beta) and basic fibroblast growth factor (bFGF) with monolayer cultures of rabbit articular chondrocytes were examined. Both agonists resulted in a synergistic increase in prostanoid production, to levels higher than the maximal level for either protein alone. The synergy was time- and dose-dependent, was augmented in serum-free medium, and was blocked by indomethacin or a polyclonal antibody to IL-1 beta. The proteins had opposite effects on cell growth, and IL-1 beta completely blocked the mitogenic effect of bFGF. Pretreatment of cells with IL-1 beta induced a down-regulation in the number of the bFGF high-affinity receptors. Pretreatment of cells with bFGF increased the number of IL-1 receptors, which was dependent on messenger RNA synthesis.

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J E Chin, C A Hatfield, R F Krzesicki, W F Herblin. 1991. Interactions between interleukin-1 and basic fibroblast growth factor on articular chondrocytes. Effects on cell growth, prostanoid production, and receptor modulation.. https://doi.org/10.1002/art.1780340309

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