Transmembrane currents in frog olfactory cilia.

S J Kleene; R C Gesteland

doi:10.1007/bf01868593

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Transmembrane currents in frog olfactory cilia.

S J Kleene·R C Gesteland

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Abstract

We have measured transmembrane currents in intact single cilia from frog olfactory receptor neurons. A single cilium on a neuron was sucked into a patch pipette, and a high-resistance seal was formed near the base of the cilium. Action potentials could be induced by applying suction or a voltage ramp to the ciliary membrane. A transient current was seen in some cells on stimulation with odorants. After excision from the cell, most of the cilia showed increased conductance in a bath containing cAMP, indicating that the cytoplasmic face of the ciliary membrane was accessible to the bath. The estimated resistance of a single cilium was surprisingly low.

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Action Potentials Animals Bucladesine Cilia Cyclic AMP Egtazic Acid Electric Conductivity Electrophysiology Membrane Potentials Microelectrodes Neurons Odorants Olfactory Mucosa Rana pipiens Journal Article Research Support, U.S. Gov't, P.H.S.

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S J Kleene, R C Gesteland. 1991. Transmembrane currents in frog olfactory cilia.. https://doi.org/10.1007/bf01868593

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