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The porcine tumor necrosis factor-encoding genes: sequence and comparative analysis.

P Kuhnert·C Wüthrich·E Peterhans·U Pauli
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Abstract

We have cloned and sequenced a 10.22-kb fragment of the genomic locus of the porcine tumor necrosis factor-encoding genes, TNF-alpha and TNF-beta. A liver genomic DNA library, partially digested with Sau3AI, was cloned into the phage lambda EMBL4 and screened with a porcine TNF-alpha cDNA probe. Analysis showed that both the TNF-alpha and TNF-beta genes were present on the cloned fragment. In addition, the cloned fragment contained about 2 kb of repetitive sequences 5' to the TNF-beta gene. The TNF genes are arranged in a tandem repeat, as is the case for the human, mouse and rabbit TNF genes. The comparison of both genes with their human homologues displayed a considerable degree of conservation (80%), suggesting an equal evolution rate.

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AnimalsBase SequenceCloning, MolecularDNAExonsGene Expression RegulationIntronsMolecular Sequence DataRepetitive Sequences, Nucleic AcidRestriction MappingSequence Homology, Nucleic AcidSwineTumor Necrosis Factor-alphaJournal ArticleResearch Support, Non-U.S. Gov't

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P Kuhnert, C Wüthrich, E Peterhans, U Pauli. 1991-06-30. The porcine tumor necrosis factor-encoding genes: sequence and comparative analysis.. https://doi.org/10.1016/0378-1119(91)90075-m

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