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Preparative high-performance liquid chromatography applied to peptide synthesis.

T F Gabriel·J Michalewsky·J Meienhofer
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Abstract

A variety of protected synthetic peptides were purified by high-performance liquid chromatography on pre-packed silica gel columns. The compounds varied in protecting groups, amino acid composition and molecular weight. Flow-rates of two to four column volumes per hour were employed, with resultant back pressure of less than 150 p.s.i. A typical column load of 0.2-5 g was purified in 2-5 h.

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Amino Acid SequenceChromatography, High Pressure LiquidGelsMolecular WeightPeptidesSilicon DioxideTime FactorsJournal Article

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T F Gabriel, J Michalewsky, J Meienhofer. 1976-12-22. Preparative high-performance liquid chromatography applied to peptide synthesis.. https://doi.org/10.1016/s0021-9673(00)87786-9

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