[Optimal chips for megabase DNA sequencing].

P A Pevzner; Iu P Lysov; K R Khrapko; A V Beliavskiĭ; V L Florent'ev; A D Mirzabekov

PubMed · 1881398

[Optimal chips for megabase DNA sequencing].

P A Pevzner·Iu P Lysov·K R Khrapko·A V Beliavskiĭ·V L Florent'ev·A D Mirzabekov

Abstract

The SHOM method (Sequencing by Hybridization with Oligonucleotide Matrix) developed in 1988 is a new approach to nucleic acid sequencing by hybridization to a octanucleotide matrix composed of an array of immobilized oligonucleotides. The original matrix proposed for sequencing by SHOM had to contain at least 65,536 octanucleotides. The present work describes a new family of matrices for sequencing, which allows one to reduce the number of synthesized oligonucleotides 5-15 times without essentially decreasing the resolving power of the method.

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Base Sequence DNA Molecular Sequence Data Nucleic Acid Hybridization English Abstract Journal Article

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P A Pevzner, Iu P Lysov, K R Khrapko, A V Beliavskiĭ, V L Florent'ev, A D Mirzabekov. [Optimal chips for megabase DNA sequencing].. https://pubmed.ncbi.nlm.nih.gov/1881398/

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