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PubMed · 1881693

[Clostridium difficile: a typing method for epidemiological studies].

G Miscopein·M Delmée·A Sedallian
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Abstract

One hundred and twenty two strains of Clostridium difficile were studied: 33 strains were isolated from stools of neonates or children, 89 cases of antibiotic associated diarrhea. The strains were identified by biochemical tests and gaz liquid chromatography of the fermentation products. A typing scheme for Clostridium difficile were proposed by using a polyacrylamide gel electrophoresis of whole cell proteins. We studied the correlation between this typing and the serogrouping scheme based on slide agglutination with a rabbit antisera and previously described by Delmée. The serogrouping defines 10 groups, PAGE allows the differentiation of 12 profiles, with a good correlation between the two methods for some serogroups except for the heterogenous serogroup A.

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Bacterial ProteinsBacterial Typing TechniquesChromatography, GasClostridioides difficileElectrophoresis, Polyacrylamide GelHumansIn Vitro TechniquesJournal Article

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G Miscopein, M Delmée, A Sedallian. 1991. [Clostridium difficile: a typing method for epidemiological studies].. https://pubmed.ncbi.nlm.nih.gov/1881693/

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