Complement C5a receptor assay for high throughput screening.

S R Harris; R K Garlick; J J Miller; H N Harney; P J Monroe

doi:10.3109/10799899109066393

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PubMed · 1886076

Complement C5a receptor assay for high throughput screening.

S R Harris·R K Garlick·J J Miller·H N Harney·P J Monroe

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Abstract

The complement C5a receptor on U937 cells, a human histiocytic lymphoma cell line, stimulated with dibutyryl-cAMP have been stabilized for at least 3 months at a dilute, ready to use concentration. [125I]-Bolton Hunter labeled C5a, (recombinant, human) has been prepared by reverse phase HPLC to 2200 Ci/mmol. Using a filtration binding assay the Kd from receptor saturation analysis is 10-40 pM and there are 50,000-100,000 receptor sites per cell. These reagents have permitted the development of a reliable, reproducible and convenient drug screening assay, in kit format, for compounds acting at the C5a receptor.

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S R Harris, R K Garlick, J J Miller, H N Harney, P J Monroe. 1991. Complement C5a receptor assay for high throughput screening.. https://doi.org/10.3109/10799899109066393

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