A microassay for proteolytic activity.

J J Plantner

doi:10.1016/0003-2697(91)90307-f

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A microassay for proteolytic activity.

J J Plantner

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Abstract

A quantitative procedure for measuring proteolytic activity, utilizing azoalbumin as substrate, has been developed for use in microtiter plates. An enzyme-linked immunosorbent assay reader is used to measure absorbance. The procedure is sensitive, as well as being both rapid and economical. It is particularly convenient for measuring large numbers of samples, such as fractions from column chromatography.

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Chromatography, Gel Enzyme-Linked Immunosorbent Assay Metalloendopeptidases Microchemistry Peptide Hydrolases Spectrophotometry Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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J J Plantner. 1991-05-15. A microassay for proteolytic activity.. https://doi.org/10.1016/0003-2697(91)90307-f

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