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The existence of triphosphorylated 5'-ends in virus-specific RNA isolated from SV-40 transformed cells.

G P Georgiev·O P Samarina·V L Mantieva
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Abstract

The question about the nature of promoters in the transcriptional units containing SV-40 sequences in transformed cells was analyzed. It was found that the pulse-labeled RNA hybridizing to SV-40 DNA contains small but significant amounts of triphosphorylated 5'-ends detected as pppGp in alkaline hydrolyzates of this RNA. In another series of experiments the fragments of RNA containing triphosphorylated 5'-ends about 100 nucleotides in length have been isolated by hydroxyapatite chromatography. Some of them form hybrids with SV-40 DNA. The conclusion is drawn that at least some of SV-40 promoters are used for transcription initiation in SV-40 transformed cells.

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Alkaline PhosphataseCell LineCell NucleusCell Transformation, NeoplasticDNA, ViralEscherichia coliNucleic Acid HybridizationOligoribonucleotidesRNA, ViralSimian virus 40Journal Article

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G P Georgiev, O P Samarina, V L Mantieva. 1976. The existence of triphosphorylated 5'-ends in virus-specific RNA isolated from SV-40 transformed cells.. https://doi.org/10.1007/bf00423230

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