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A method for preparing analytically pure sodium dithionite. Dithionite quality and observed nitrogenase-specific activities.

C E McKenna·W G Gutheil·W Song
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Abstract

Sodium dithionite (Na2S2O4) is widely used as a reductant in biochemical studies, but has not been available in its pure form. A convenient, detailed procedure is given for the recrystallization of commercial dithionite from 0.1 M NaOH-methanol under anaerobic conditions. Twice-recrystallized dithionite had a purity of 99 +/- 1% by UV spectroscopy (A315) and elemental analysis. The influence of dithionite quality on the apparent reduction activities of the nitrogenase components (Av1 and Av2) from Azotobacter vinelandii was investigated.

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CrystallizationNitrogenaseOxidation-ReductionSpectrophotometry, UltravioletSulfatesJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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C E McKenna, W G Gutheil, W Song. 1991-09-02. A method for preparing analytically pure sodium dithionite. Dithionite quality and observed nitrogenase-specific activities.. https://doi.org/10.1016/0304-4165(91)90082-r

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