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PubMed · 1903260

HIV-1 indicator cell lines.

A Akrigg·G W Wilkinson·S Angliss·P J Greenaway
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Abstract

A simple quantitative bioassay for infectious HIV-1 has been developed. The assay is based on adherent CD4+ HeLa cell lines stably transfected with episomal vectors carrying the Escherichia coli beta-galactosidase gene under the control of the HIV long terminal repeat (LTR) promoter. HIV infection of these cell lines transactivates the LTR promoter inducing beta-galactosidase production. Infected cells and virus foci can be stained dark blue by the addition of the chromogenic substrate X-Gal. Alternatively, a readily automatable quantitative enzyme assay can be performed on the infected cultures. Because of its simplicity the bioassay may be useful for routine quantification of HIV-infected cultures, plaque purification, virus neutralization studies and for the screening of antiviral agents.

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Biological AssayCD4 AntigensCloning, MolecularGalactosidesHIV-1HeLa CellsHumansIndolesPlasmidsTransfectionbeta-GalactosidaseJournal ArticleResearch Support, Non-U.S. Gov't

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A Akrigg, G W Wilkinson, S Angliss, P J Greenaway. 1991. HIV-1 indicator cell lines.. https://doi.org/10.1097/00002030-199102000-00004

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