V lambda and J lambda-C lambda gene segments of the human immunoglobulin lambda light chain locus are separated by 14 kb and rearrange by a deletion mechanism.

G Combriato; H G Klobeck

doi:10.1002/eji.1830210627

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V lambda and J lambda-C lambda gene segments of the human immunoglobulin lambda light chain locus are separated by 14 kb and rearrange by a deletion mechanism.

G Combriato·H G Klobeck

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Abstract

We have cloned a region of 124 kb of the human immunoglobulin lambda light chain locus on chromosome 22 encompassing seven V lambda and seven J-C lambda gene segments. No further C lambda gene segment was found in a region of 35 kb downstream of C lambda 7, which encodes the Ke+Oz- isotype. The C lambda proximal V lambda gene segment V lambda III. 1 is located 14.5 kb upstream of C lambda 1. The five sequenced V lambda genes have the same transcriptional orientation as the J-C lambda gene segments which is likely to be true for the majority of the V lambda gene segments in the human lambda locus and which suggests a deletion mechanism for DNA rearrangement. This is supported by hybridization of V lambda gene probes to germ-line and rearranged DNA from lambda light chain-producing cell lines. Sequences of 23 cDNA clones allow to establish a V lambda subgroup classification based on nucleic acid sequence data and an estimate of the J-C lambda usage.

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Amino Acid Sequence Base Sequence Chromosome Deletion Chromosome Mapping Cloning, Molecular DNA Gene Rearrangement Genes, Immunoglobulin Humans Immunoglobulin Constant Regions Immunoglobulin Joining Region Immunoglobulin Light Chains Immunoglobulin Variable Region Immunoglobulin lambda-Chains Molecular Sequence Data Mutation Pseudogenes Journal Article Research Support, Non-U.S. Gov't

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G Combriato, H G Klobeck. 1991. V lambda and J lambda-C lambda gene segments of the human immunoglobulin lambda light chain locus are separated by 14 kb and rearrange by a deletion mechanism.. https://doi.org/10.1002/eji.1830210627

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