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A specific fluorogenic assay for N-acetylgalactosamine-4-sulphatase activity using immunoadsorption.

D A Brooks·G J Gibson·P A McCourt·J J Hopwood
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Abstract

A method combining immune capture and enzyme detection by fluorochemistry has been developed for the diagnostic assay of N-acetylgalactosamine-4-sulphatase (4-sulphatase). The procedure uses a monoclonal antibody 4-S 4.1 to immunoadsorb 4-sulphatase specifically from complex protein samples containing other sulphatases, and 4-methylumbelliferyl sulphate to detect captured 4-sulphatase. The assay provides an accurate and simple method for the diagnosis of Maroteaux-Lamy syndrome (Mucopolysaccharidosis type VI).

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Antibodies, MonoclonalChondro-4-SulfataseHumansHymecromoneImmunoenzyme TechniquesImmunosorbent TechniquesMucopolysaccharidosis IVSpectrometry, FluorescenceJournal ArticleResearch Support, Non-U.S. Gov't

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D A Brooks, G J Gibson, P A McCourt, J J Hopwood. 1991. A specific fluorogenic assay for N-acetylgalactosamine-4-sulphatase activity using immunoadsorption.. https://doi.org/10.1007/bf01804381

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