Isolation and characterization of a cDNA clone encoding the anti-viral protein from Phytolacca americana.

Q Lin; Z C Chen; J F Antoniw; R F White

doi:10.1007/bf00037047

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Isolation and characterization of a cDNA clone encoding the anti-viral protein from Phytolacca americana.

Q Lin·Z C Chen·J F Antoniw·R F White

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Abstract

Phytolacca anti-viral protein (PAP) was purified from Phytolacca leaves and the N-terminal was sequenced. A cDNA library was made from mRNAs isolated from Phytolacca leaves and cDNA clones for PAP were identified using oligonucleotide probes derived from the N-terminal amino acid sequence. The PAP-cDNA clone was sequenced from both directions. The predicted amino acid sequence of PAP was compared with the amino acid sequences of other ribosome-inactivating proteins. The identities of these proteins to PAP ranged from 29 to 38%, and a region was found in each with a sequence similar to the PAP sequence (AIQMVSEAARFKYI). Southern blot analysis indicates that PAP is encoded by a multi-gene family.

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Amino Acid Sequence Base Sequence Blotting, Southern DNA Probes Molecular Sequence Data Multigene Family N-Glycosyl Hydrolases Oligonucleotides Plant Proteins Plants Ribosome Inactivating Proteins, Type 1 Sequence Homology, Nucleic Acid Journal Article Research Support, Non-U.S. Gov't

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Q Lin, Z C Chen, J F Antoniw, R F White. 1991. Isolation and characterization of a cDNA clone encoding the anti-viral protein from Phytolacca americana.. https://doi.org/10.1007/bf00037047

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