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Rapid method for preparing a beta-glucan-specific sensitive fraction from Limulus (Tachypleus tridentatus) amebocyte lysate.

T Kitagawa·I Tsuboi·S Kimura·Y Sasamoto
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Abstract

By separating Limulus amebocyte lysate by cation-exchange chromatography with an SP-Toyopearl 650C column, a fraction insensitive to endotoxin, yet specifically sensitive to beta-glucan, was successfully obtained in the unadsorbed portion. This fraction showed beta-glucan dose-dependent clotting enzyme activity, although no sensitivity to endotoxin. This beta-glucan-dependent reaction showed no interference in the presence of endotoxin, with the fraction also showing sensitivity towards various kinds of beta-glucan, i.e. curdlan, pachyman, laminaran and lichenan. The sensitivity towards curdlan was approximately 10(-10) g/ml.

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AnimalsBacterial ToxinsChromatography, LiquidElectrophoresis, Polyacrylamide GelGlucansHorseshoe CrabsPolysaccharidesPolysaccharides, BacterialSensitivity and SpecificitySpectrophotometry, Ultravioletbeta-GlucansJournal Article

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T Kitagawa, I Tsuboi, S Kimura, Y Sasamoto. 1991-06-14. Rapid method for preparing a beta-glucan-specific sensitive fraction from Limulus (Tachypleus tridentatus) amebocyte lysate.. https://doi.org/10.1016/0378-4347(91)80331-6

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