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PubMed · 1931024

A rapid method for purifying PCR products for direct sequence analysis.

M H Kalnoski·M F McCoy-Haman·G F Hollis
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Abstract

An HPLC approach for purification and sequencing of double-stranded DNA obtained directly from a PCR is described. This simple and reliable procedure has several advantages; the DNA fragment is rapidly eluted (less than 7 minutes), requires no organic cleanup, produces several hundred bases of sequence and is sensitive enough to obtain DNA sequence from a single 100-microliters PCR. This method is demonstrated by sequencing tumor necrosis factor alpha (TNF alpha) gene amplified from mouse tail DNA.

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AnimalsBase SequenceChromatography, High Pressure LiquidDNAMiceMolecular Sequence DataPolydeoxyribonucleotidesPolymerase Chain ReactionTime FactorsJournal Article

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M H Kalnoski, M F McCoy-Haman, G F Hollis. 1991. A rapid method for purifying PCR products for direct sequence analysis.. https://pubmed.ncbi.nlm.nih.gov/1931024/

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