Decrease of GABA-immunoreactive neurons in the amygdala after electrical kindling in the rat.

P M Callahan; J M Paris; K A Cunningham; P Shinnick-Gallagher

doi:10.1016/0006-8993(91)90361-x

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Decrease of GABA-immunoreactive neurons in the amygdala after electrical kindling in the rat.

P M Callahan·J M Paris·K A Cunningham·P Shinnick-Gallagher

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Abstract

The present study was designed to investigate the effects of electrical kindling in vivo on GABA immunoreactivity (GABA-IR) of the lateral and basolateral amygdaloid nuclei 2-6 months post-stimulation. Male Sprague-Dawley rats were implanted with bipolar electrodes in the basolateral nucleus and stimulated once per day until 3-5 stage 5 seizures were observed. Coronal sections containing the amygdala were processed for GABA-IR using the contralateral side of the brain. Results indicate that, in comparison to controls, fully kindled animals showed a significant decrease in total number of GABA-IR amygdala neurons. Decreases in GABA-positive punctate structures surrounding unlabeled pyramidal cells were also observed, but not quantified. The present data suggest that epileptogenesis of the amygdala is associated with a significant reduction of GABA-IR in the lateral and basolateral areas throughout the contralateral amygdaloid nucleus.

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3,3'-Diaminobenzidine Amygdala Animals Electric Stimulation Electrodes In Vitro Techniques Kindling, Neurologic Male Neurons Rats Rats, Inbred Strains Stereotaxic Techniques gamma-Aminobutyric Acid Journal Article Research Support, U.S. Gov't, P.H.S.

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P M Callahan, J M Paris, K A Cunningham, P Shinnick-Gallagher. 1991-08-02. Decrease of GABA-immunoreactive neurons in the amygdala after electrical kindling in the rat.. https://doi.org/10.1016/0006-8993(91)90361-x

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