New method for isolating and quantifying intermediate and beta-very-low-density lipoprotein cholesterol.

R L Wikinski; L E Schreier; S B Rosental

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PubMed · 1934464

New method for isolating and quantifying intermediate and beta-very-low-density lipoprotein cholesterol.

R L Wikinski·L E Schreier·S B Rosental

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Abstract

We describe a new method, useful to clinical laboratories, for assessing intermediate density (IDL) or beta-very-low-density (beta-VLDL) lipoprotein cholesterol. The technique involves selective precipitation properties of the qualitative Wieland and Seidel post-electrophoretic method that immobilizes IDL and beta-VLDL in the beta-zone of an agarose slide (Clin Chem 1973;19:1139-41). In our method, we separate low-density lipoprotein (LDL) in a second electrophoretic step, in which LDL moves toward the anode, and then quantify the cholesterol of the above lipoproteins remaining in the precipitate band at the beta-zone. Replicate within-run precision (CV) of 15 aliquots of a sera pool was 10.1%. The correlation with sequential ultracentrifugation of 30 samples was r = 0.96 (P less than 0.001). Serum reference values for 30 normal individuals are 57 +/- 7.0 mg/L. Seven phenotype III hyperlipoproteinemic patients had the highest concentrations of IDL or beta-VLDL cholesterol in serum, 1620 +/- 346 mg/L.

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Chemical Precipitation Cholesterol Cholesterol, VLDL Electrophoresis, Agar Gel Humans Hyperlipoproteinemia Type III Lipoproteins Reference Values Triglycerides Ultracentrifugation Comparative Study Journal Article Research Support, Non-U.S. Gov't

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R L Wikinski, L E Schreier, S B Rosental. 1991. New method for isolating and quantifying intermediate and beta-very-low-density lipoprotein cholesterol.. https://pubmed.ncbi.nlm.nih.gov/1934464/

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