Phage T4 expression vector: protection from proteolysis.

B S Singer; L Gold

doi:10.1016/0378-1119(91)90558-s

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Phage T4 expression vector: protection from proteolysis.

B S Singer·L Gold

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Abstract

We have developed an efficient method for the expression of heterologous genes during infection by T4, a bacteriophage known to inhibit the proteolytic systems of Escherichia coli. This system enables us to clone genes in a plasmid expression vector and move them readily into T4. We have used lacZ as a reporter gene to show that both plasmid and phage exhibit low basal expression or high-level expression under the control of a T7 promoter. This system promises a possible solution to the problem of degradation and/or toxicity of overproduced proteins.

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Electrophoresis, Polyacrylamide Gel Escherichia coli Gene Expression Genes, Viral Genetic Vectors Hydrolysis Plasmids Promoter Regions, Genetic Recombination, Genetic T-Phages Transcription, Genetic Journal Article Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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B S Singer, L Gold. 1991-09-30. Phage T4 expression vector: protection from proteolysis.. https://doi.org/10.1016/0378-1119(91)90558-s

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