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PubMed · 1937033

High-efficiency yeast artificial chromosome fragmentation vectors.

W J Pavan·P Hieter·D Sears·A Burkhoff·R H Reeves
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Abstract

Chromosome fragmentation vectors (CFVs) are used to create deletion derivatives of large fragments of human DNA cloned as yeast artificial chromosomes (YACs). CFVs target insertion of a telomere sequence into the YAC via homologous recombination with Alu repetitive elements. This event results in the loss of all YAC sequences distal to the site of integration. A new series of CFVs has been developed. These vectors target fragmentation to both Alu and LINE human repetitive DNA elements. Recovery of deletion derivatives is ten- to 20-fold more efficient with the new vectors than with those described previously.

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Chromosomes, FungalGene LibraryGenetic VectorsGenome, HumanHumansRestriction MappingTransformation, GeneticJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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W J Pavan, P Hieter, D Sears, A Burkhoff, R H Reeves. 1991-09-30. High-efficiency yeast artificial chromosome fragmentation vectors.. https://doi.org/10.1016/0378-1119(91)90576-w

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