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Defective interfering particles with covalently linked [+/-]RNA induce interferon.

P I Marcus·M J Sekellick
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Abstract

Defective interfering (DI) particles of vesicular stomatitis virus which contain covalently linked complementary [+]message and [-]anti-message RNA as a single-stranded ribonucleoprotein complex within the particle, are extremely efficient inducers of interferon. A single particle can induce a quantum yield of interferon. A single molecule of double-stranded RNA presumed to form, at least in part, on entry into the cell is thought to induce interferon synthesis. Conventional [-]RNA DI particles with the same polypeptide composition as [+/-]RNA DI particles fail to induce interferon.

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Antibodies, ViralAntigen-Antibody ReactionsCell LineCell SurvivalCycloheximideDactinomycinDefective VirusesHot TemperatureInterferonsNucleic Acid ConformationRNA, ViralTemplates, GeneticUltraviolet RaysVesicular stomatitis Indiana virusViral InterferenceJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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P I Marcus, M J Sekellick. 1977-04-28. Defective interfering particles with covalently linked [+/-]RNA induce interferon.. https://doi.org/10.1038/266815a0

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