Protein hydration in aqueous solution.

G Otting; E Liepinsh; K Wüthrich

doi:10.1126/science.1948083

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PubMed · 1948083

Protein hydration in aqueous solution.

G Otting·E Liepinsh·K Wüthrich

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Abstract

High-resolution proton nuclear magnetic resonance studies of protein hydration in aqueous solution show that there are two qualitatively different types of hydration sites. A well-defined, small number of water molecules in the interior of the protein are in identical locations in the crystal structure and in solution, and their residence times are in the range from about 10(-2) to 10(-8) second. Hydration of the protein surface in solution is by water molecules with residence times in the subnanosecond range, even when they are located in hydration sites that contain well-ordered water in the x-ray structures of protein single crystals.

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Animals Cattle Crystallography Magnetic Resonance Spectroscopy Models, Molecular Oxytocin Pancreas Protein Conformation Solutions Trypsin Inhibitors Water X-Ray Diffraction Journal Article Research Support, Non-U.S. Gov't

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G Otting, E Liepinsh, K Wüthrich. 1991-11-15. Protein hydration in aqueous solution.. https://doi.org/10.1126/science.1948083

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