A new quantitative assay for the determination of complement activity.

V Stefanski; H G Ruppel

doi:10.1016/0165-2478(91)90081-k

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A new quantitative assay for the determination of complement activity.

V Stefanski·H G Ruppel

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Abstract

The suitability of the algal assay and the hemolysis method for the determination of complement activity was investigated with regard to reproducibility and quantification. It was shown that the day-to-day reproducibility is higher in the algal assay than in the hemolysis method in test tubes. The lysis of algal cells depends on serum concentrations. The extensive linear relationship between cytotoxic activity and serum concentration prevails at lysis rates between 20 and 80%. Therefore, it is often sufficient to measure the lysis rate at one serum concentration only. The algal assay enables the investigation of changes in complement activity, even if the changes are not expected to be drastic.

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Animals Complement Hemolytic Activity Assay Complement System Proteins Cytotoxicity Tests, Immunologic Euglena gracilis Female Guinea Pigs Male Reproducibility of Results Comparative Study Journal Article Research Support, Non-U.S. Gov't

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V Stefanski, H G Ruppel. 1991. A new quantitative assay for the determination of complement activity.. https://doi.org/10.1016/0165-2478(91)90081-k

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