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Leucocyte function in rats with naturally occurring gingivitis.

K Ohura·M Shinohara·K Ogata·A Nishiyama·M Mori
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Abstract

The severity of gingivitis in relation to abnormal macrophage chemotaxis and superoxide (O2-) production was investigated in rats with naturally occurring gingivitis (plaque-susceptible rats). Macrophage chemotaxis was measured by the membrane filter method; zymosan-activated serum was used as a chemo-attractant. O2- production was measured by the reduction of cytochrome c method. Opsonized zymosan, phorbol myristate acetate and calcium ionophore A23187 were used as stimulants. The macrophage chemotaxis and O2- production in the plaque-susceptible rats with deep pockets were significantly lower than those in the susceptible rats with shallow pockets or the normal group. In addition, there was a negative correlation between the severity of gingivitis in plaque-susceptible rats and macrophage chemotaxis or O2- production. These findings suggest that impairement of the host defence mechanisms due to the reduction in macrophage chemotaxis and O2- production is related to the progression of periodontal disease in plaque-susceptible rats.

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AnimalsCell Migration InhibitionChemotaxisDental PlaqueDisease SusceptibilityGingivitisMacrophagesPeriodontal PocketRatsRats, Inbred StrainsSuperoxidesJournal Article

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K Ohura, M Shinohara, K Ogata, A Nishiyama, M Mori. 1990. Leucocyte function in rats with naturally occurring gingivitis.. https://doi.org/10.1016/0003-9969(90)90154-3

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