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A chemically-defined medium for organotypic slice cultures.

C M Annis·J Edmond·R T Robertson
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Abstract

Organotypic slice cultures provide an excellent system for the analysis of study of the molecular mechanisms of this development necessitates the use of a chemically defined culture medium. We report here the development of a medium, EOL1 defined medium, designed specifically for this purpose. Cultures of both cerebral cortex and basal forebrain demonstrate that this defined medium allows a high degree of cytoarchitectural maintenance while promoting neural metabolism and process outgrowth.

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AnimalsCerebral CortexCulture MediaImmunohistochemistryNeurotransmitter AgentsOrgan Culture TechniquesRatsRats, Inbred StrainsJournal Article

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C M Annis, J Edmond, R T Robertson. 1990. A chemically-defined medium for organotypic slice cultures.. https://doi.org/10.1016/0165-0270(90)90072-n

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