[Pasteurella multocida bacteriophage adsorption].

Abstract

Studied was the adsorption of 8 strains of Pasteurella multocida bacteriophages presenting three groups of phages isolated in Bulgaria. With the bacteriophages of groups I and II the adsorption in broth in the first 10 minutes amounted to 50-65%, and at the 80 the minute reached its maximum value--98-99%. With the bacteriophages of group III adsorption started with a lower percent and by the 140th minute reached 96-97%. It was by 60 min slower as compared with the adsorption with group I and II phages. In a veronal-medinal buffer the adsorption of all investigated phages of the three groups set in with a prolonged time of 60 min as compared with the time elapsed using broth. At the cross testing of adsorption the phages of group I were adsorbed by the hosts of the other two groups to a maximum level, however, the phages of groups II and III were adsorbed to a negligible extent on the other hosts (except for the adsorption on their homologous one). The difference established in the adsorption for a 60 minutes time so far as the phages of group III were concerned was sufficiently persuasive to consider this group as an individual one by this feature too. Adsorption appeared as a subsidiary taxonomic characteristic in the classification of Pasteurella multocida bacteriophages.