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PubMed · 1987490

Man-made antibodies.

G Winter·C Milstein
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Abstract

Monoclonal antibodies can now be genetically engineered and endowed with new properties. In the future, gene technology could enable antigen-binding fragments to be made by exploiting repertoires of variable domain genes derived from immunized animals and expressed in bacteria. How readily can this approach be extended to production of 'in vitro' repertoires of variable domain genes, and obviate the immunization of animals?

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AnimalsAntibodies, MonoclonalAntibody DiversityAntigensBinding Sites, AntibodyGene RearrangementGenes, ImmunoglobulinGenetic EngineeringHumansHybridomasImmunizationImmunoglobulin Variable RegionMacromolecular SubstancesMiceMolecular StructureMutagenesisProtein ConformationRatsJournal ArticleReview

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G Winter, C Milstein. 1991-01-24. Man-made antibodies.. https://doi.org/10.1038/349293a0

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