PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 1991534

Discrimination between nonhyperactivated and classical hyperactivated motility patterns in human spermatozoa using computerized analysis.

L J Burkman
View original source ↗Export metadata ↓

Abstract

The objective identification of human hyperactivated (HA) motility has been controversial. The present study defines new criteria for automatic sorting of all HA patterns that are consistent with the classical descriptions. Sperm from fertile men were prepared in Ham's F-10 medium. Based on the author's previously published slow-motion method, 342 sperm were selected that represented the non-HA, circling high-curvature, thrashing, star, and helical patterns. Automatic sorting for HA was achieved with the use of three criteria: linearity less than or equal to 65 and velocity greater than or equal to 100 microns/s and head displacement greater than or equal to 7.5 microns. Additional criteria were determined for identifying each HA class separately. Whether using the new or old method, the incidence of HA is significantly associated with multiple fertilization endpoints.

Explore related subjects

AutoanalysisHumansIn Vitro TechniquesMaleReference ValuesSoftwareSperm MotilityVideo RecordingComparative StudyJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

L J Burkman. 1991. Discrimination between nonhyperactivated and classical hyperactivated motility patterns in human spermatozoa using computerized analysis.. https://pubmed.ncbi.nlm.nih.gov/1991534/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed1992

[Significance of the automated blood count].

Modern hematology depends on rapid and accurate determination of quantitative and qualitative blood counts. Fully automated analyzers based on the principles of flow cytometry have replaced the traditional blood smear in the routine laboratory. They count the number of red cells, platelets and leukocytes with high precision. In addition, they divide the cells into different populations according to their physical or chemical properties. This population distribution pattern mimics a "differential" blood smear. Normality can be assessed with a high degree of accuracy, rapidly and reproducibly. The qualitative changes frequently provide pointers to the final diagnosis. In case of abnormalities the reports are flagged. A microscopic analysis should be added according to internal laboratory guidelines. It clarifies pathological findings and is essential in the search for specific abnormalities such as malaria. This underlines the need for exchange of information between physician and laboratory.

Autoanalysis↗
PubMed1992

Fast liquid chromatography for the determination of drugs in plasma and combination with liquid-solid extraction in a fully automated system.

Fast liquid chromatography was applied to the assay of several drugs in plasma. Short columns, 3.3-4 cm long, packed with C18 material, 3 microns particle size, were used. The peaks were little subject to extra-column band-broadening because the investigated drugs were eluted with high capacity factors in order to obtain an adequate separation from plasma components. The main influences on efficiency were the response time of the detector and the solvent composition of the injected sample. Conventional apparatus was used. A fully automated analytical system combining liquid-solid extraction via disposable extraction columns and fast liquid chromatography on a small-dimensioned 3 microns particle size column is described for the assay of drugs in plasma. Automation was accomplished by using the Automatic Sample Preparation with Extraction Columns system.

Autoanalysis↗